The Correlation Between DsRed mRNA Levels and Transient DsRed Protein Expression in Plants Depends on Leaf Age and the 5' Untranslated Region.
Identifieur interne : 000087 ( Main/Exploration ); précédent : 000086; suivant : 000088The Correlation Between DsRed mRNA Levels and Transient DsRed Protein Expression in Plants Depends on Leaf Age and the 5' Untranslated Region.
Auteurs : Julia Jansing [Allemagne] ; Johannes F. Buyel [Allemagne]Source :
- Biotechnology journal [ 1860-7314 ] ; 2019.
Descripteurs français
- KwdFr :
- ARN messager (génétique), Agrobacterium tumefaciens (génétique), Biosynthèse des protéines (génétique), Feuilles de plante (génétique), Protéines luminescentes (génétique), Protéines recombinantes (génétique), Régions 5' non traduites (génétique), Régions promotrices (génétique) (génétique), Régulation de l'expression des gènes végétaux (génétique), Tabac (génétique), Végétaux génétiquement modifiés (génétique).
- MESH :
English descriptors
- KwdEn :
- 5' Untranslated Regions (genetics), Agrobacterium tumefaciens (genetics), Gene Expression Regulation, Plant (genetics), Luminescent Proteins (genetics), Plant Leaves (genetics), Plants, Genetically Modified (genetics), Promoter Regions, Genetic (genetics), Protein Biosynthesis (genetics), RNA, Messenger (genetics), Recombinant Proteins (genetics), Tobacco (genetics).
- MESH :
Abstract
The yield of recombinant proteins in plants determines their economic competitiveness as a production platform compared to microbes and mammalian cells. The promoter, untranslated regions (UTRs) and codon usage can all contribute to the yield, but potential interactions among these components have not been examined in detail. Here the effect of two promoters (35SS and nos) and four 5'UTRs on the spatiotemporal expression of DsRed mRNA and the accumulation of DsRed protein during transient expression in tobacco (Nicotiana tabacum) mediated by Agrobacterium tumefaciens is investigated. The authors found that the mRNA levels peaked 2-3 days post-infiltration (dpi), and rapidly declined thereafter, whereas DsRed protein was first detected after ≈3 days and concentrations continued to increase until at least 5 dpi. This temporal decoupling of mRNA and protein expression was strongest in the older leaves, which also produced the lowest DsRed yields. The accumulation of DsRed linearly correlated with mRNA levels in all but the youngest leaves, where more DsRed was synthesized per mRNA molecule. This was the case for both promoters, although the nos promoter had a higher protein/mRNA ratio than the 35SS promoter. Furthermore, the type of 5'UTR affected DsRed protein accumulation by 50% starting from similar levels of mRNA. The authors concluded that DsRed mRNA levels are not the limiting factor for DsRed protein expression in plants, but that translation-associated processes such as initiation, elongation, and release are bottlenecks that should be addressed in future studies.
DOI: 10.1002/biot.201800075
PubMed: 29701331
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Correlation Between DsRed mRNA Levels and Transient DsRed Protein Expression in Plants Depends on Leaf Age and the 5' Untranslated Region.</title><author><name sortKey="Jansing, Julia" sort="Jansing, Julia" uniqKey="Jansing J" first="Julia" last="Jansing">Julia Jansing</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1,Aachen, 52074, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1,Aachen, 52074</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation></author><author><name sortKey="Buyel, Johannes F" sort="Buyel, Johannes F" uniqKey="Buyel J" first="Johannes F" last="Buyel">Johannes F. Buyel</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1,Aachen, 52074, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1,Aachen, 52074</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6,Aachen, 52074, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6,Aachen, 52074</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:29701331</idno><idno type="pmid">29701331</idno><idno type="doi">10.1002/biot.201800075</idno><idno type="wicri:Area/Main/Corpus">000178</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000178</idno><idno type="wicri:Area/Main/Curation">000178</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000178</idno><idno type="wicri:Area/Main/Exploration">000178</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The Correlation Between DsRed mRNA Levels and Transient DsRed Protein Expression in Plants Depends on Leaf Age and the 5' Untranslated Region.</title><author><name sortKey="Jansing, Julia" sort="Jansing, Julia" uniqKey="Jansing J" first="Julia" last="Jansing">Julia Jansing</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1,Aachen, 52074, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1,Aachen, 52074</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation></author><author><name sortKey="Buyel, Johannes F" sort="Buyel, Johannes F" uniqKey="Buyel J" first="Johannes F" last="Buyel">Johannes F. Buyel</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1,Aachen, 52074, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1,Aachen, 52074</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6,Aachen, 52074, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6,Aachen, 52074</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation></author></analytic><series><title level="j">Biotechnology journal</title><idno type="eISSN">1860-7314</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>5' Untranslated Regions (genetics)</term><term>Agrobacterium tumefaciens (genetics)</term><term>Gene Expression Regulation, Plant (genetics)</term><term>Luminescent Proteins (genetics)</term><term>Plant Leaves (genetics)</term><term>Plants, Genetically Modified (genetics)</term><term>Promoter Regions, Genetic (genetics)</term><term>Protein Biosynthesis (genetics)</term><term>RNA, Messenger (genetics)</term><term>Recombinant Proteins (genetics)</term><term>Tobacco (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>Agrobacterium tumefaciens (génétique)</term><term>Biosynthèse des protéines (génétique)</term><term>Feuilles de plante (génétique)</term><term>Protéines luminescentes (génétique)</term><term>Protéines recombinantes (génétique)</term><term>Régions 5' non traduites (génétique)</term><term>Régions promotrices (génétique) (génétique)</term><term>Régulation de l'expression des gènes végétaux (génétique)</term><term>Tabac (génétique)</term><term>Végétaux génétiquement modifiés (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>5' Untranslated Regions</term><term>Luminescent Proteins</term><term>RNA, Messenger</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Gene Expression Regulation, Plant</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Promoter Regions, Genetic</term><term>Protein Biosynthesis</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Agrobacterium tumefaciens</term><term>Biosynthèse des protéines</term><term>Feuilles de plante</term><term>Protéines luminescentes</term><term>Protéines recombinantes</term><term>Régions 5' non traduites</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes végétaux</term><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The yield of recombinant proteins in plants determines their economic competitiveness as a production platform compared to microbes and mammalian cells. The promoter, untranslated regions (UTRs) and codon usage can all contribute to the yield, but potential interactions among these components have not been examined in detail. Here the effect of two promoters (35SS and nos) and four 5'UTRs on the spatiotemporal expression of DsRed mRNA and the accumulation of DsRed protein during transient expression in tobacco (Nicotiana tabacum) mediated by Agrobacterium tumefaciens is investigated. The authors found that the mRNA levels peaked 2-3 days post-infiltration (dpi), and rapidly declined thereafter, whereas DsRed protein was first detected after ≈3 days and concentrations continued to increase until at least 5 dpi. This temporal decoupling of mRNA and protein expression was strongest in the older leaves, which also produced the lowest DsRed yields. The accumulation of DsRed linearly correlated with mRNA levels in all but the youngest leaves, where more DsRed was synthesized per mRNA molecule. This was the case for both promoters, although the nos promoter had a higher protein/mRNA ratio than the 35SS promoter. Furthermore, the type of 5'UTR affected DsRed protein accumulation by 50% starting from similar levels of mRNA. The authors concluded that DsRed mRNA levels are not the limiting factor for DsRed protein expression in plants, but that translation-associated processes such as initiation, elongation, and release are bottlenecks that should be addressed in future studies.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29701331</PMID><DateCompleted><Year>2019</Year><Month>06</Month><Day>14</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1860-7314</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><Issue>3</Issue><PubDate><Year>2019</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Biotechnology journal</Title><ISOAbbreviation>Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>The Correlation Between DsRed mRNA Levels and Transient DsRed Protein Expression in Plants Depends on Leaf Age and the 5' Untranslated Region.</ArticleTitle><Pagination><MedlinePgn>e1800075</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/biot.201800075</ELocationID><Abstract><AbstractText>The yield of recombinant proteins in plants determines their economic competitiveness as a production platform compared to microbes and mammalian cells. The promoter, untranslated regions (UTRs) and codon usage can all contribute to the yield, but potential interactions among these components have not been examined in detail. Here the effect of two promoters (35SS and nos) and four 5'UTRs on the spatiotemporal expression of DsRed mRNA and the accumulation of DsRed protein during transient expression in tobacco (Nicotiana tabacum) mediated by Agrobacterium tumefaciens is investigated. The authors found that the mRNA levels peaked 2-3 days post-infiltration (dpi), and rapidly declined thereafter, whereas DsRed protein was first detected after ≈3 days and concentrations continued to increase until at least 5 dpi. This temporal decoupling of mRNA and protein expression was strongest in the older leaves, which also produced the lowest DsRed yields. The accumulation of DsRed linearly correlated with mRNA levels in all but the youngest leaves, where more DsRed was synthesized per mRNA molecule. This was the case for both promoters, although the nos promoter had a higher protein/mRNA ratio than the 35SS promoter. Furthermore, the type of 5'UTR affected DsRed protein accumulation by 50% starting from similar levels of mRNA. The authors concluded that DsRed mRNA levels are not the limiting factor for DsRed protein expression in plants, but that translation-associated processes such as initiation, elongation, and release are bottlenecks that should be addressed in future studies.</AbstractText><CopyrightInformation>© 2018 The Authors. Biotechnology Journal Published by Wiley-VCH Verlag GmbH & Co. KGaA.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jansing</LastName><ForeName>Julia</ForeName><Initials>J</Initials><Identifier Source="ORCID">https://orcid.org/0000-0003-2339-0083</Identifier><AffiliationInfo><Affiliation>Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1,Aachen, 52074, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Buyel</LastName><ForeName>Johannes F</ForeName><Initials>JF</Initials><Identifier Source="ORCID">https://orcid.org/0000-0003-2361-143X</Identifier><AffiliationInfo><Affiliation>Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1,Aachen, 52074, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6,Aachen, 52074, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>269110</GrantID><Agency>Ideas: European Research Council</Agency><Country></Country></Grant><Grant><GrantID>125-600164</GrantID><Agency>Fraunhofer-Gesellschaft</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>05</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Biotechnol J</MedlineTA><NlmUniqueID>101265833</NlmUniqueID><ISSNLinking>1860-6768</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020121">5' Untranslated Regions</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008164">Luminescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C413663">fluorescent protein 583</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020121" MajorTopicYN="N">5' Untranslated Regions</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008164" MajorTopicYN="N">Luminescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014176" MajorTopicYN="N">Protein Biosynthesis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">design of experiments</Keyword><Keyword MajorTopicYN="N">molecular farming</Keyword><Keyword MajorTopicYN="N">promoter/5′UTR combination</Keyword><Keyword MajorTopicYN="N">real time PCR</Keyword><Keyword MajorTopicYN="N">transient expression</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>02</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>04</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>4</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>6</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>4</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29701331</ArticleId><ArticleId IdType="doi">10.1002/biot.201800075</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>District de Cologne</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Aix-la-Chapelle</li></settlement></list><tree><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Jansing, Julia" sort="Jansing, Julia" uniqKey="Jansing J" first="Julia" last="Jansing">Julia Jansing</name></region><name sortKey="Buyel, Johannes F" sort="Buyel, Johannes F" uniqKey="Buyel J" first="Johannes F" last="Buyel">Johannes F. Buyel</name><name sortKey="Buyel, Johannes F" sort="Buyel, Johannes F" uniqKey="Buyel J" first="Johannes F" last="Buyel">Johannes F. Buyel</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000087 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000087 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= AgrobacTransV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:29701331
|texte= The Correlation Between DsRed mRNA Levels and Transient DsRed Protein Expression in Plants Depends on Leaf Age and the 5' Untranslated Region.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:29701331" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AgrobacTransV1
| This area was generated with Dilib version V0.6.38. |  |